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. 2022 Aug 12;25:460–471. doi: 10.1016/j.bioactmat.2022.07.030

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Fig. 3 — The HA@SDF-1α hydrogel promoted chemotaxis of HMSCs and HUVECs. (a, b) SDF-1α stimulated a significant increase in the expression level of CXCR4 with increased SDF-1α concentration as verified with qRT-PCR analysis and western blotting. (c) SDF-1α could be released in a sustained manner. (d, e) qRT-PCR analysis and western blotting showed an enhanced expression level of CXCR4 when cells were cultured on an HA@SDF-1α hydrogel. (f–h) Scratch wound healing assay and transwell migration assay were conducted to test the migration effect of the HA@SDF-1α hydrogel (scale bar = 500 or 100 μm). Data are presented as mean ± SD of triplicate experiments, **P < .01, ***P < .001, ****P < .0001.