Fig. 5.

M2D-Exosomal miRNA-5106 enhanced angiogenesis in vitro. (a) The miR-5106 level was significantly higher after HA@M2D-Exos hydrogel treatment. (b) HUVECs were treated with PBS or transfected with AgomiR-5106 or AntagomiR-5106. The miR-5106 expression level was assessed using qRT-PCR analysis. (c) Proliferation-related protein Cyclin D1 and Cyclin D3 level were assessed with western blotting. (d, f) The effect of miR-5106 on HUVEC proliferation was assessed with CCK-8 and EdU assay (scale bar = 100 μm). (e) The proliferation state was assessed with a cell cycle assay. (g, h) HUVEC tube formation ability was significantly higher after miR-5106 transfection (scale bar = 200 μm). Data are presented as mean ± SD of triplicate experiments, **P < .01, ***P < .001.