Table 4. Single Point Mutation of MsrA02.

entry	single point mutation	enzyme conc. (gL–1)a,b	(R)-1a ee%
1	C23S	1.0	26
		10	67
		40	80
2	C25Sc	0.23	<1
		2.3	8
		9.2	21
3	C44S	1.0	9
		10	99
		40	>99
4	C68Sc	0.23	>99
		2.3	>99
		9.2	>99
5	C176S	1.0	4
		10	26
		40	36
6	Wild type	1.0	>99

^aEnzyme used as CFE.

^bgL^–1 of protein concentration calculated from 10, 100, and 400 μM.

^cPurified enzyme was used.