Versatility of SEQUIN for bulk and single-cell RNA-seq
Overview of experimental models and next-generation sequencing that generate transcriptomic datasets. Two paths of data generation are shown: “bulk” populations of cells from homogenized organisms, tissues, or cultured cells or single-cell suspension after dissociation. The first path leads to averaged gene expression values of the transcriptomes, while the second creates a transcriptome library of each cell. Either sequencing data format can be input to SEQUIN for analysis, which is a free and fully featured R/Shiny application for both types of data.