Figure 6.
The Quantifier captures quantitative changes in rodent behaviors
(A) Raster plots showing the frame-wise categorizations by RatA of behavior after saline or amphetamine injection. Plots at the left (baseline) show responses to the first exposure to amphetamine across increasing doses (baseline; 0.32, 1.0, 3.2, and 5.6 mg/kg, i.p.); Plots at the right show responses to increasing doses of amphetamine after repeated amphetamine treatment (post treatment; 21 days). Animals in the saline group received repeated saline throughout. Color bars: behavioral categories (intensities indicate the behavioral probabilities). x axis: time (each tick is 10-s); y axis: each row represents an individual rat. These same colors are used to indicate behavioral categories in (B)–(K).
(B–D) The dose-response curves of rearing intensity (B), locomotion counts (C), and head movement duration (D) following the first exposure to amphetamine or saline (baseline). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 (unpaired t test between saline and amphetamine groups).
(E) Dose-response curves for head movement duration at baseline and after repeated amphetamine exposure (post treatment, i.e., sensitization). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 (unpaired t test between baseline and post treatment groups).
(F–H) Within subject comparison of rearing intensity (F), locomotion distance (G) and the head movement duration (H) in response to the first 0.32 mg/kg amphetamine at baseline and after repeated amphetamine treatment. ∗p < 0.05; ∗∗p < 0.01 (paired t test).
(I–K) Dose-response curves of the intensity and counts of body grooming (I and J) and the duration of face grooming (K) in in rats repeatedly treated with saline.
(L) Raster plots showing the frame-wise categorizations by MouseH for a younger (32-week) or an older (80-week) mouse. Color bars: behavioral categories (intensities indicate the behavioral probabilities). x axis: time (in seconds); y axis: different random 2-min time window. Multiple (>100) 2-min time windows were randomly selected from a 4-h recording for each mouse, and those containing wheel-running behavior (targeted time window) were sorted out. Ten targeted time windows for each mouse were randomly selected for analysis.
(M) The running vigor of the two mice in (L). ∗∗p < 0.01 (unpaired t test).
All error bars represent standard error of the mean.