Fig. 5.

Lysosomal function is restored by glycyrrhizin. a–b Infection with H. pylori SS1 strain (MOI 100) was performed for 4 h followed by glycyrrhizin (GLZ) (200 µM) exposure for 4 h. a Lysosomal membrane integrity was monitored by Acridine Orange (AO) staining in a fluorescence microscope. Briefly, cells were incubated with 10 μg/ml of acridine orange (15 min) and examined. The mean fluorescence intensity of the red signal was determined and graphically represented. Scale bar: 10 μm. b Live cell imaging of drug-treated, infected and control cells was done with LysoTracker Red incubation (100 nM, 30 min) to label lysosomes and mean fluorescence intensity was assessed under the confocal microscope. Scale bar: 10 μm. Fold change was quantified and graphs were generated using GraphPad Prism 5 and represented as mean ± SEM (n = 3); Significance was calculated by one-way ANOVA; *p < 0.05, **p < 0.01