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. Author manuscript; available in PMC: 2023 Apr 11.
Published in final edited form as: J Mol Cell Cardiol. 2022 Apr 9;168:13–23. doi: 10.1016/j.yjmcc.2022.04.002

Fig. 3.

Fig. 3.

Reproducibility of FLT-FRET HTS Hits. Normalized plot of the effects of 10 μM Hit on FRET to A-CaM (A) or A-DPc10 (B) exceeding 4SD in 2 of 3 screens. SR was exposed to no H2O2 (black for individual values and green for mean values±SD) and H2O2 treatment (purple for individual values and pink for mean values±SD). The first 18 Hits altered both A-CaM and A-DPc10 FRET, Hits 19–37 only altered A-CaM FRET, and Hits 38–42 altered A-DPc10 FRET. These groups were assigned based on the Fig. 2 representation, sectors being indicated in parentheses for each compound. A-CaM FRETcontrol values of SR exposed to no H2O2 and plus H2O2 treatment were 0.11 ± 0.01 and 0.09 ± 0.01, respectively. A-DPc10 FRETcontrol values of SR exposed to no H2O2 and plus H2O2 treatment were 0.1 ± 0.02 and 0.11 ± 0.04, respectively. Chemical structures are shown in Supplementary Figs. 2 and 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)