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. Author manuscript; available in PMC: 2023 Apr 11.

Published in final edited form as: J Mol Cell Cardiol. 2022 Apr 9;168:13–23. doi: 10.1016/j.yjmcc.2022.04.002

Table 1.

Number (#) of Hits and Hit reproducibility for the 4SD threshold.

A-CaM FRET	No H₂O₂			H₂O₂ treatment

	Plate 1	Plate 2	Plate 3	Plate 1	Plate 2	Plate 3
# of Hits 20 min	70	24	44	81	51	42
# of Hits 120 min	70	54	54	105	24	60
% of Repeated Hits in 2 plates^a	52.9	63.0	66.7	37.1	83.3	63.3
% of Repeated Hits in 3 plates^a	44.3	57.4	57.4	18.1	79.1	31.2
A-DPc10 FRET
# of Hits 20 min	35	47	25	35	25	31
# of Hits 120 min	26	63	28	28	31	31
% of Repeated Hits in 2 plates^a	50	36.5	78.6	75.0	71.0	54.8
% of Repeated Hits in 3 plates^a	46.2	19.0	42.9	50	45.2	45.2

^a

Data for 120 min incubation with compound.