Table 2.
Primer probe gene symbol | Taqman assay gene expression | Gene name |
---|---|---|
ACTB | Hs01060665_g1 | Actin beta |
DNMT1 (original, OR) | Hs00154749_m1 | DNA (cytosine-5-)-methyltransferase 1 Transcript variant 1 (DNMT1b), 2 (DNMT1a), and variants 3,4 |
DNMT1 CP (custom probe) | AIFAT65 | DNA (cytosine-5-)-methyltransferase 1\u00B0F: 5′-CGTCTAGAAAACGGGAACCAAGCAAG-3′ R: 5′-TCTAATCCCAGTTACTTGGGAGGCTG-3′ |
DNMT3A | Hs01027166_m1 | DNA methyltransferase 3 alpha |
GAD1 | Hs01065893_m1 | glutamate decarboxylase 1 (full length, multiple transcripts) |
GAD1 (variant GAD67) | Hs01065886_m1 | glutamate decarboxylase 1 (truncated form GAD67) |
GAD1 (variant GAD25) | Hs00247564_m1 | glutamate decarboxylase 1 (truncated form GAD25) |
The differing portions of the gene covered in the original and these duplicate TaqMan probes for the same main gene are further specified in the information under the Gene Name column in the table. The GAD1 full length TaqMan probe contains the transcripts for the variants GAD67, GAD25, and multiple other transcripts.