Figure 5.

The microbiome differed between the four centres. (A) Taxonomic summary of OTUs on phylum level, n = 10 from each centre. (B) Total numbers of OTUs for each centre. Graphs show mean ± SD. Statistical significance was assessed by pairwise Student’s t-tests for independent samples (New York versus Odense: t = 0.29, P = 1; New York versus Hamburg, t = 4.76, P = 0.001; New York versus Münster: t = −4.55, P = 0.004; Hamburg versus Odense: t = −4.48, P = 0.002; Hamburg versus Münster: t = −9.65, P < 0.001; Münster versus Odense: t = −4.88, P = 0.001; n = 10 in each centre). (C) Principal coordinate analysis (PCoA) of the family composition. Clusters of each centre are highlighted with an ellipse. Statistical testing was performed with an analysis of similarities (R = 0.78, P < 0.001, n = 40). (D) qPCR analysis for SFB presence in mice from the four different centres. Expression levels were normalized to corresponding SFB levels in mice from Odense. Log₂ fold changes are shown as mean ± SD. SFB were not detectable in the New York cohort. Statistical significance was assessed by Student’s t-tests for independent samples (Hamburg: t₍₁₄₎ = 2.23, P = 0.04, n = 16; Münster: t₍₁₆₎ = 2.58; P = 0.02, n = 18).