Table 2.
Target | Primary antibody | Antibody dilution | Clone | Antigen retrieval | Incubation settings |
---|---|---|---|---|---|
PLP | Biorad #MCA839G | 1:1000 | Monoclonal | Citrate pH6 microwave | 1 h RT |
CD8 | Dako #IS623 | 1:5 | Monoclonal | Citrate pH6 autoclave | 1 h RT |
CD3 | Dako #A0452 | 1:100 | Polyclonal | Tris-EDTA pH 9 autoclave | 1 h RT |
Nurr1 | Abcam #ab41917 | 1:1500 | Monoclonal | Tris-EDTA pH 9 autoclave | ON 4°C |
Nurr1 | Santacruz #Sc376984 | 1:50 (IF) | Monoclonal | Tris-EDTA pH 9 autoclave | ON 4°C |
NeuN | Millipore #MAB377 | 1:400 | Monoclonal | Citrate pH6 autoclave | 1 h RT |
NeuN | Abcam #ab177487 | 1:200 (IF) | Monoclonal | Tris-EDTA pH 9 autoclave | ON 4°C |
GAD | Millipore #ab1511 | 1:200 | Polyclonal | Citrate pH6 autoclave | 1 h RT |
TMEM119 | Sigma #HPA051870 | 1:200 (IF) | Monoclonal | Tris-EDTA pH 9 autoclave | ON 4°C |
GFAP | Abcam #ab4674 | 1:1000 (IF) | Monoclonal | Tris-EDTA pH 9 autoclave | ON 4°C |
IF = immune-fluorescence.