Skip to main content
. 2023 Mar 17;5(2):fcad072. doi: 10.1093/braincomms/fcad072

Table 2.

Antibodies used in staining procedures presented in this article

Target Primary antibody Antibody dilution Clone Antigen retrieval Incubation settings
PLP Biorad #MCA839G 1:1000 Monoclonal Citrate pH6 microwave 1 h RT
CD8 Dako #IS623 1:5 Monoclonal Citrate pH6 autoclave 1 h RT
CD3 Dako #A0452 1:100 Polyclonal Tris-EDTA pH 9 autoclave 1 h RT
Nurr1 Abcam #ab41917 1:1500 Monoclonal Tris-EDTA pH 9 autoclave ON 4°C
Nurr1 Santacruz #Sc376984 1:50 (IF) Monoclonal Tris-EDTA pH 9 autoclave ON 4°C
NeuN Millipore #MAB377 1:400 Monoclonal Citrate pH6 autoclave 1 h RT
NeuN Abcam #ab177487 1:200 (IF) Monoclonal Tris-EDTA pH 9 autoclave ON 4°C
GAD Millipore #ab1511 1:200 Polyclonal Citrate pH6 autoclave 1 h RT
TMEM119 Sigma #HPA051870 1:200 (IF) Monoclonal Tris-EDTA pH 9 autoclave ON 4°C
GFAP Abcam #ab4674 1:1000 (IF) Monoclonal Tris-EDTA pH 9 autoclave ON 4°C

IF = immune-fluorescence.