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. 2023 Apr 11;5:24. doi: 10.1186/s42523-023-00244-w

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The effect of ROM treatment on luminal microbiota colonization. Boxplots show comparisons of jejunal luminal microbial alpha diversity between ROM and Ctrl groups on day 70 based on observed richness (A), phylogenetic diversity (B), Shannon diversity (C) and inverse Simpson (InvSimpson) (D), as well as caecal luminal microbial diversity between two groups on day 44 with indices of observed richness (E) and Shannon diversity (F). Differences in alpha diversity between groups were evaluated by nonparametric Wilcoxon rank sum test. Principal coordinate analysis (PCoA) plots for jejunal luminal microbiota (G) and caecal luminal microbiota (H) at ASV level both on day 70 based on unweighted UniFrac. Significance of the difference between ROM and Ctrl groups was assessed using PERMANOVA. The percentages at the axes indicate the variation explained. Linear discriminant analysis (LDA) Effect size (LEfSe) of differentially abundant jejunal taxa between ROM and Ctrl groups on day 70 (I). Cladogram of caecal differentially abundant taxa between ROM and Ctrl pigs on day 70 according to LEfSe (J). Blue and yellow colours represent control (Ctrl) and treated (ROM) groups, respectively