FIGURE 3.

APN inhibits IR‐induced oxidative stress and inflammatory response by up‐regulating miR‐21; A, Inhibition of miR‐21 significantly reduced plasma APN levels in the lower extremities of IR mice; B, Inhibition of miR‐21 significantly reduced the expression level of APN in gastrocnemius of IR mice; C, Injection of APN significantly increased the expression level of miR‐21 in gastrocnemius of IR mice; D, Inhibition of miR‐21 significantly reversed the changes in plasma creatinine and oxidative stress factor levels induced by APN injection in IR mice; E, Inhibition of miR‐21 significantly reversed the changes in the levels of inflammatory factors in gastrocnemius of IR mice induced by APN injection; &&P < 0.01 versus sham + miR‐NC, *P < 0.05 and **P < 0.01 versus IR + miR‐NC, ##P < 0.01 versus IR + APN + miR‐21 inhibitor