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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Methods Mol Biol. 2023;2626:135–150. doi: 10.1007/978-1-0716-2970-3_7

Table 2.

Antibodies useful for visualizing the fusome and/or microtubules in dividing ovarian germ cells

Antigen (Host
Species)		 Vendor
(Catalog)		 Description Localization
Hts (mouse) DSHB (1B1) Monoclonal antisera Fusome
LaminC (mouse) DSHB (LC28.26) Monoclonal antisera, useful for identifying cap cells Nuclear envelope
α-Spectrin (mouse) DSHB (3A9) Monoclonal antisera Fusome
α-Tubulin-AF555 (mouse) Millipore (#05-829-AF555) Directly conjugated monoclonal antisera (clone DM1A), can be purchased with AlexaFluor-555 or AlexaFluor-488 Fusome, mitotic spindle
GFP (chicken) Abcam (ab13970) Polyclonal antisera, useful for marking GFP-tagged transgenes (see Table 1) Variable
dsRed (rabbit) Clontech / Takara (632496) Polyclonal antisera, useful for marking mCherry- and RFP-tagged transgenes (see Table 1) Variable

DSHB Developmental Studies Hybridoma Bank