Figure 3.
DEPDC1B promotes the proliferation of EOC cells. (A) Protein levels of DEPDC1B in human normal ovarian cell line IOSE-80, and the EOC cell lines OVCAR3 and SKOV3 were determined by western blot assays. (B) Efficiency of DEPDC1B upregulation was confirmed by western blot in stable DEPDC1B overexpression (DEPDC1BOE) and control (DEPDC1BCtrl) OVCAR3 and SKOV3 cells. (C, D) Representative images from colony formation assay in DEPDC1BOE and DEPDC1BCtrl OVCAR3 (C) and SKOV3 (D) cells. (E, F) EdU cell proliferation assay in DEPDC1BOE and DEPDC1BCtrl OVCAR3 (E) and SKOV3 (F) cells. Data shown are the mean ± SEM of three independent experiments. *, P < 0.05, **, P < 0.01.
