Table 1.

List of genes with the most significant difference in expression levels; i.e. those that showed more than 30-fold up-regulated in low-light-acclimated cells as compared with high light

Gene Number (Phatr3)	Log2 Fold-change LL vs. HL	Fold-change in LL vs. HL	P-adj value (FDR)	NCBI BLAST Hit	NCBI Conserved Protein Domains	Signal Peptide (predicted)	Transmembrane (predicted)	Anchored to Membrane (predicted)	Targeted to Chloroplast (predicted)
EG01529	9.76	870	1.58 e-146	Predicted Protein	-	+	+	-	inconclusive
J47715	7.67	203	2.34 e-146	Predicted Protein	-	-	-	inconclusive	-
J43123	7.36	164	1.43 e-174	Predicted Protein	-	+	+	-	+
EG00707	6.46	88	7.66 e-188	Predicted Protein	-	+	+	-	+
J37655	6.45	87	7.43 e-113	Predicted Protein	-	+	+	inconclusive	+
J50052	5.39	42	2.04 e-127	Predicted Protein	Kinase (ABC1 family)	+	-	-	+

All these genes were targeted for RNAi individually. Phatr3 – gene identification numbers from third annotation of genome (protists.ensembl.org); LL – low-light intensity of 20-μmol photons m⁻²s⁻¹; HL – high-light intensity of 940-μmol photons m⁻²s⁻¹; P-adj value (FDR) – P value of transcriptome results adjusted to false discovery rate <0.05; NCBI - The National Center for Biotechnology Information database; BLAST Hit – Results from The Basic Local Alignment Search Tool of NCBI; (+) indicates presence; (−) indicates absence. (n = 3; P < 0.05)