Fig. 6.

The effect of p.S396D on the motor kinetics of prestin. Stimulus frequency-dependent NLC was measured in OHCs isolated from WT (A), S396D-prestin (A), R130S-prestin (B), and R130S/S396D-prestin (B) homozygous mice under high (144 mM) and low (1 mM) intracellular chloride conditions using five different f₁ frequencies (195, 390, 781, 1,563, and 3,125 Hz) with the corresponding f₂ frequencies being twice as large as f₁ (i.e., 390, 780, 1,563, 3,125, 6,250 Hz). Dashed lines through data points in Left panels indicate the double-log linear regression from which P values (compared with “WT 144 mM Cl⁻” in A or “R130S 144 mM Cl⁻” in B) were determined by F-tests. “ns”, not significant (P ≥ 0.05); “*”, 0.01 ≤ P < 0.05; “**”, 0.001 ≤ P < 0.01. Relative NLC shown in Right panels allow for a clearer appreciation of differences in frequency responses (motor kinetics). Error bars in Left panels indicate SDs, while those in Right panels indicate propagated errors calculated from SEs of the original data shown in Left panels. OHCs were collected from mice at P27-63.