Figure 2.

Summary of Na⁺ sensitivity EC₅₀ values for rSlack mutants. A–D, The sodium dependence EC₅₀ values of 91 mutant constructs (including some double or triple amino acid mutants) are summarized. The Na⁺ sensitivity EC₅₀ values were estimated. *Labels the minimum EC₅₀ values of E490Q, E787Q, and E899Q mutants. The comparison of the EC₅₀ of the WT channel with the EC₅₀ of E490Q (p = 0.0009), E491Q (p = 0.001), E787Q (p = 0.0009), E899Q (p = 0.001), and D955 (p = 0.01) shows significant difference. The mutants (E373Q, D863N, E865Q, and D1196N) that did not show macroscopic currents were labeled with black circles.