Fig 4. Lack of efficacy of the AAV8-based delivery of ShK-235 in Lewis rats.

(A) AAV8 constructs injected intraperitoneally into rats, and their doses. N = 7–9 rats per group. (B) Difference (Δ) in ear thickness in rats immunized against ovalbumin and challenged with ovalbumin in one ear and with saline in the other. Each data point represents a different rat. Data from males and females are combined. (C) ShK-235 concentration in serum from rats injected with each of the different AAV8. Each serum sample was tested on 3 cells each. (D) Representative IHC images of livers from rats with DTH, showing GFP expression. The black arrows point to EGFP⁺ cells. Scale bars, 100 μm.