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. Author manuscript; available in PMC: 2023 Apr 11.
Published in final edited form as: Nature. 2022 Jun 22;606(7916):945–952. doi: 10.1038/s41586-022-04844-5

Extended Data Fig. 4. PD-1+ CD8 T cells are effector memory T cells activated in an antigen fashion.

Extended Data Fig. 4.

. a, Expression of indicated surface markers in CD8 T cells from the spinal cord (top), brain (middle) and peripheral blood (bottom) of 10–12 month old KI animals after gating on PD-1+ and PD-1− CD8 T cells. Histograms are representative of 3 independent experiment (n=6 mice). b, Median fluorescence intensity (MFI) of the indicated markers in CD44+CD62L− PD-1+ and PD-1− CD8 T cells isolated from the spinal cord (top) and blood (bottom) of 10–12 month old KI mice (2 independent experiments, n=6 (blood TIGIT and TOX), n=5 (spinal cord TIGIT and TOX), n=4 (TCF-1) individual mice/group, mean +/− SEM). p-value calculated using a two-tailed unpaired t test with Welch’s correction (spinal cord: ***p=0.0007; *p=0.045; **p=0.0066; blood: ***p=0.0004 (TOX); ***p=0.0002 (TIGIT and TCF-1). c, KI mice were crossed to OT-I mice and the offspring analyzed at 10 months of age. Dot plots from spinal cords, SC (left), and bar graphs (right) show the frequency of PD-1+ among OVA specific (gated on Vα2+Vβ5+) and non-TCR transgenic (gated on Vα2−) CD8 T cells (3 independent experiments, n=6, brain and spinal cord, and n=5, blood, individual mice; mean+/− SEM). p-value calculated using a two-tailed unpaired t test with Welch’s correction. **p=0.0031 (brain) ; ***p=0.0004 (SC); ***p=0.0003 (blood). eF=eFluor.