Figure 2:

KDM1A expression is not regulated by UVA radiation: (A, B) KDM1A protein level expression compared to normalizer Lamin A/C expression in bizine treated HaCat and FaDu after UVA exposure at doses which in parallel experiments induced significant decrease in cell viability. C-Control, B-Bizine KDM1A inhibition increases lipid peroxidation and mRNA expression of antioxidant enzymes after UVA irradiation : (C, D) Lipid peroxidation by estimating red to green shift in BODIPY^® 581/591 C11 measured in HaCaT and FaDu cells treated with bizine and exposed to UVA radiation (E-G) ROS responsive genes KLF-9, HMOX-1 and NOXA expression compared to PGK1 after KDM1A inhibition using bizine UVA exposure. RNA was harvested 4 hours after the addition UVA exposure in HaCaT cells NAC salvaged cells from KDM1A inhibition induced death (H,I) HaCaT and FaDu cells were treated with NAC, bizine or NAC and bizine for 18 hours before exposure to either 2 doses of UVA. Cell viability is expressed as a percentage of control and was assessed using cell titer blue assay. (G-I). Cell viability is expressed as a percentage of control and was assessed using cell titer blue assay. Bars represent mean ± SD (n=3). *: p<0.05