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. 2023 Apr 11;13:5891. doi: 10.1038/s41598-023-32720-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the electrophysiological properties of NPFF cells with those of GRPR cells. Data for the GRPR cells was obtained from Polgár et al¹⁸. (a) The incidence of action potential firing patterns differed between NPFF and GRPR cells. NPFF cells exhibited a greater proportion of tonic (30.8 vs. 2.6%) and initial burst firing (19.2 vs. 4.3%) cells and fewer single spike firing cells (7.7 vs. 40.2%). The incidence of delayed firing was similar between both cell types (42.3 vs. 48.7%). NPFF cells were found to have a lower rheobase (26.9 ± 20.5 v.s 68.7 ± 43.4 pA, P < 0.0001, b), action potential voltage threshold (-35.3 ± 5.4 vs. -30.0 ± 7.3 mV, P < 0.0001, c) and a greater action potential height (64.8 ± 10.2 vs. 45.2 ± 10.4 mV, P < 0.0001, d) and after-hyperpolarisation (-28.4 ± 5.2 vs. − 25.9 ± 4.9 mV, P = 0.024, e). In terms of excitatory synaptic input, sEPSC (6.86 ± 6.15 vs. 4.34 ± 5.26 Hz, P = 0.005, f) and mEPSC (2.35 ± 1.99 vs. 1.02 ± 1.34 Hz, P = 0.002, g) frequency in NPFF cells was greater than that in GRPR cells, as was the mEPSC amplitude (30.1 ± 4.1 vs. 27.1 ± 5.4 pA, P = 0.023, h). (i) The incidence of subthreshold voltage-activated currents differed between NPFF and GRPR cells. NPFF cells demonstrated a higher incidence of I_As (25.0 vs. 3.7%) and I_h (68.8 vs. 28.4%), but a lower incidence of I_Ar (62.5 vs. 95.1%). (j) The peak amplitude of the I_Ar recorded in NPFF cells was significantly lower than for GRPR cells (165.7 ± 80.3 vs. 289.7 ± 154.7, P = 0.007). All statistical comparisons made using a Mann Whitney test, except for afterhyperpolarisation, which was compared using an unpaired t test.