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. 2023 Apr 11;14:2045. doi: 10.1038/s41467-023-37873-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Loss of MCEMP1 protein expression in Mcemp1^–/– mice. Lungs were immunoblotted with MCEMP1 antibody. Blots are representative of two independent experiments. b, c Cell growth kinetics of Mcemp1^+/+ and Mcemp1^–/– peritoneal cell (PC, n = 5 per group) or bone marrow cells (BM, n = 4 per group) cultured with SCF and IL-3 for the indicated days. d Cell growth kinetics of Mcemp1^+/+ and Mcemp1^–/– KIT-positive PC isolated by magnetic beads and cultured with SCF and IL-3 for the indicated days. e, f Absolute counts of Mcemp1^+/+ and Mcemp1^–/– PC cultured with either SCF or IL-3 for the indicated days (n = 3 per group). g Schematics of SCF intranasal (i.n.) challenge of Mcemp1^+/+ and Mcemp1^–/– mice. h Representative flow cytometry plots illustrating the gating strategy to identify KIT/FcεRI double-positive mast cells; CD45⁺LIN^–KIT⁺FcεRI⁺. The percentages of KIT⁺FcεRI⁺ mast cells in the lungs of Mcemp1^+/+ or Mcemp1^–/– mice challenged with saline or SCF (n = 6-7 mice per group). Data are presented as violin plot with lines at median and quartiles and p-values were determined by two-way ANOVA with Sidak’s multiple comparison in b, c, d, e, f, h. ns, not significant.