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. 2023 Apr 11;14:2045. doi: 10.1038/s41467-023-37873-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a IPA canonical pathways enriched in Mcemp1^–/– PC versus Mcemp1^+/+ PC cultured with SCF and IL-3 for 9 days. The z-scores indicate a predicted inhibition or activation of the indicated pathway. The ratio was calculated by the number of DEGs in the indicated pathway divided by the total number of genes that map to the pathway. The p-value of overlap was calculated by the right-tailed Fisher’s Exact Test. b Heatmaps of differentially expressed genes (DEGs) of Mcemp1^–/– PC versus Mcemp1^+/+ PC. c A dot plot of comparison analysis of upstream regulators repressed in Mcemp1^–/– PC in comparison with Mcemp1^+/+ PC. The color bar indicates z-score of a predicted inhibition in the indicated upstream regulators. The size of each dot corresponds to the p-value of overlap (right-tailed Fisher’s Exact Test). d, e Representative immunofluorescence images of Mcemp1^+/+ or Mcemp1^–/– PC cultured with SCF and IL-3 for the indicated days and stained with anti-phospho-JNK antibody (green), anti-phospho-MITF antibody (red) or Hoechst 33342 (blue). Scale bar, 20 μm. The average percentage of p-JNK or p-MITF positive cells per slide (n = 4–9 slides) were quantified. Data are representative of two independent experiments. f Expressions of Ccne1, Ccna1, Ccnb1, and Ccnb2 in Mcemp1^+/+ or Mcemp1^–/– PC. KIT-positive PC were isolated and cultured with SCF and IL-3 for 6 days and stimulated with 30 ng/ml SCF and further cultured for 12 h (n = 9-18 per group). g Schematic illustration of SCF-KIT and IgE-FcεRI signal transduction in mast cells. The linker for activation of T cells (LAT) is an essential adaptor for FcεRI receptor that induces IgE-mediated mast cell activation. In parallel, MCEMP1 acts as a critical adaptor for KIT receptor that promotes SCF-mediated mast cell proliferation. Data are presented as violin plot with lines at median and quartiles and p-values were determined by two-tailed unpaired t-test in d–f. ns, not significant.