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. 2023 Apr 11;14:2045. doi: 10.1038/s41467-023-37873-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematics of ovalbumin (OVA)-sensitized and challenged chronic asthma model. Mcemp1^+/+ and Mcemp1^–/– mice were sensitized with saline or 50 μg OVA via intraperitoneal (i.p.) injection and challenged with saline or 20 μg OVA via intranasal (i.n.) instillation on the indicated days. b Representative images of hematoxylin and eosin staining for histological analysis of lungs. B, bronchiole; A, alveoli; V, blood capillary. Scale bar, 100 μm. c Percentages and absolute numbers of eosinophils in bronchoalveolar lavage (BAL) were determined by manual counting and by differential counts on cytospin slides (n = 3 for saline group, n = 4 for OVA group). d Percentages and absolute numbers of eosinophils and Ly-6C⁺ inflammatory monocytes in the lungs were determined by flow cytometry (n = 3 per group). e Average numbers of toluidine blue positive mast cells per slide (n = 3 slides per group) were quantified by ImageJ. f Quantification of Masson’s trichrome staining (n = 2 slides per group) by Image-Pro 10. g Schematics of BMMC adoptive transfer and OVA-induced chronic asthma model. h Percentages and/or absolute numbers of BAL eosinophils, lung CD45⁺ leukocytes, eosinophils and neutrophils were determined by flow cytometry (n = 6 for saline group. n = 3-4 for OVA group). Data are presented as violin plots with lines at median and quartiles and p-values were determined by two-way ANOVA with Sidak’s multiple comparison in c, d, e, f or by one-way ANOVA with Tukey’s multiple comparison in h. ns, not significant.