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. 2023 Apr 11;6:393. doi: 10.1038/s42003-023-04737-x

Fig. 2. Direct reprogramming of human PBMCs into iMSCs using five episomal vectors expressing OCT4, BCL-XL, MYC, KLF4, and SOX9 (5F).

Fig. 2

a Representative images of human PBMCs and iMSCs 14 days after reprogramming with five factors (5F). Scale bar represents 100 μm. b Changes in the percentage of cells expressing the MSC markers CD73 and CD90 as measured by flow cytometry of 5F-transfected PBMCs over time. c, d Flow cytometry plots of typical MSC marker expression (CD29, CD73, CD90, CD166) at 4 weeks after reprogramming. n = 3 biologically independent samples for time point. e Blood cell markers (CD45 and CD34) were assessed 4 weeks after transfection of reprogramming factors. f iMSCs significantly inhibited T-cell proliferation after 3 days of co-culture with PBMCs. **P = 0.0007. Error bars indicate standard deviation. n = 3 biologically independent samples for each group.