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. 2023 Apr 11;6:393. doi: 10.1038/s42003-023-04737-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a The bar graph showing the methylation levels of all sites, promoters, exons, and intron regions from 5F, 4FnoO, and 4FnoK iMSCs. n = 2 biologically independent samples for each group. b The methylation levels of the TSS region. n = 2 biologically independent samples for each group. c The percentage of differentially methylated CpGs (DMCs) between 5F and 4FnoO iMSCs annotated within the promoter, exon, intron, and intergenic regions shown in the pie chart. d The average methylation levels surrounding the TSSs (−5000 to +5000 bp) in 5F, 4FnoO, and 4FnoK iMSCs. e Hierarchical clustering and heatmap analysis of 13,974 DMCs. f The bar plot showing the log₂() normalized read counts from RNA-seq. n = 3 biologically independent samples for each group. *P < 0.05; error bars indicate standard deviation.