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. 2023 Apr 11;13:5879. doi: 10.1038/s41598-023-32716-z

Figure 1.

Figure 1

Schematic illustration of the overall workflow. (a) Chemical fixation and pre-sectioning starting from a large tissue. (b) High-pressure freezing (HPF) on a grid with parallel bars using 2-methylpentane. A Perfect Loop (Diatome, DZ8) is used to transfer the sliced sample to the grid. (c) CryoFIB milling with CSEI-based localization. The incident ion beam should be approximately along the direction of the grid bars in order to limit the milling within the space between neighoring bars. (d) Typical SEM image of the sample with multiple lamellae (yellow boxes) prepared on one tissue block.