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. 2023 Apr 11;13:5879. doi: 10.1038/s41598-023-32716-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CSEI-based localization on a liver slice. (a) Selected region (black box) for CSEI on a liver tissue slice, shown by low-magnification SEM. (b) Large window (orange box in the top image, under FIB view) milled for CSEI (bottom image). (c) Magnified CSEI area on the surface created by cryoFIB milling of (b) showing high contrast features of liver cells. The cell membrane (white arrow) and multiple organelles (colorful boxes and corresponding magnified images shown on the right) in liver cells were observed. All images of CSEI were acquired using Crossbeam 550 (ZEISS Microscopy).