a Schematic of CBD-IL-12, composed of the p35 and p40 subunits fused to the CBD from von Willebrand factor domain A3. b Volumes of RM9 subcutaneous tumors in sygeneic C57Bl/6 mice over time with treatment with vehicle, anti-PD-1 (clone 29 F.1A12) 200 Μg by intraperitoneal injection every 5 days, or CBD-IL-12 25 Μg by intravenous injection every 5 days starting on day 0. n = 7 mice in vehicle and CBD-IL-12 treated groups and n = 8 mice in anti-PD1 treated group. p < 0.0001 at day 9 and 12. Bars represent mean with SEM. P-values are derived from two-way ANOVA with Dunnett’s multiple comparisons test, ns not significant. c Uniform Manifold Approximation and Projection (UMAP) plots of different immune cell subsets (top) from single-cell RNA-seq (scRNA-seq) analysis of five RM9 tumors each aggregated from mice treated with vehicle or CBD-IL-12. UMAP plots colored with gene expression of immune cell subset-specific markers for pan-monocytes/macrophages, M1 and M2 polarized marcophages, conventional type 1 dendritic cells (cDC1), natural killer (NK) cells, and T helper type 1 (Th1) cells. d Plots showing the frequency of specific immune cell populations (relative to CD45 + immune cells) identified by scRNA-seq analysis including CD4+ and CD8+ T cells, Th1 (Infg+Tbx21+) and Th2 (cMAF+Gata3+) cells, Ly6C+/− monocytes/macrophages (Ly6C+/−Adgre1+), M1 macrophages (CD80+CD86+INOS2+), M2 macrophages (CD163+Mrc1+cMAF+), cDC1 (XCR1+IRF8+), conventional type 2 dendritic cells (cDC2, CD1+IRF4+), migratory CD103+ dendritic cells (Itgae+), eosinophils (SiglecF+), neutrophils (Ly6G+), and NK cells (Klrb1c+Ncr1+) in tumors treated with vehicle or CBD-IL-12. Volcano plots showing differential gene expression in (e) tumor cells and (f) T cells from RM9 tumors of mice treated CBD-IL-12 relative to those treated with vehicle. FC fold change, FDR false discovery rate. Source data are provided in the Source Data file.