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. 2023 Mar 29;14:1166246. doi: 10.3389/fphys.2023.1166246

FIGURE 4.

FIGURE 4

Effect of melatonin on GABAergic miniature IPSCs in RVLM projecting PVN neurons (A) Representative tracings from a labeled RVLM projecting PVN neuron show that mIPSCs recorded during control, application of 100 nM melatonin, washout, and application of 20 μM bicuculline. Note that bicuculline completely eliminated mIPSCs. (B,C), Cumulative probability plot analysis of mIPSCs of the same neuron showing the distribution of the interevent interval (B) and peak amplitude (C) during control, melatonin application, and washout. Melatonin shifted the distribution curve of amplitude of mIPSCs to the right (p < 0.05; Kolmgorov—Smirnov test) without changing the distribution of the interevent-interval. (D,E), Summary data show the effect of 100 nM melatonin on the frequency (D) and amplitude (E) of mIPSCs of 8 labeled PVN neurons. Data are presented as means ± SEM (*p < 0.05 compared with the control; Kruskal–Wallis ANOVA, followed by Dunn’s post hoc test). MLT, melatonin.