Table 1.
Summary of selected Parabasalia-like contigs of interest derived from the macaque fecal metatranscriptome
| Contig name | Annotation | Pathway/function | T. vaginalis hit % identity (protein level) | Mean abundance (RPM) | Rank (ranked by abundance of all Parabasalia-like contigs |
|---|---|---|---|---|---|
| NODE_1480 | Glutamate dehydrogenase | Amino acid catabolism | 56.1 | 6031 | 1 |
| NODE_1122 | Phosphoenol pyruvate carboxykinase | Gluconeogenesis | 55.9 | 4187 | 3 |
| NODE_1126 | Starch branching enzyme | Starch synthesis | 48.0 | 3211 | 4 |
| NODE_791 | Fructose-1,6-bisphosphate aldolase | Glycolysis | 65.7 | 3006 | 6 |
| NODE_638a | Glyceradehyde-3-phosphate dehydrogenase | 85.3 | 2791 | 8 | |
| NODE_1712 | Phosphoglucomutase/phosphomannomutase | 42.4 | 2136 | 12 | |
| NODE_24 | Pyruvate:ferredoxin oxidoreductase BII | Hydrogenosome metabolism | 74.3 | 875 | 45 |
| NODE_952 | Ornithine carbamoyltransferase family protein | Arginine dihydrolase pathway | 89.8 | 758 | 53 |
| NODE_539 | Malic enzyme | Hydrogenosome metabolism | 83.6 | 289 | 200 |
| NODE_134 | Aldehyde oxidase and xanthine dehydrogenase | Purine catabolism | 82.8 | 281 | 207 |
| NODE_2461 | Glucosamine-6-phosphate isomerase family protein | GlcNAc catabolism | 49.8 | 265 | 222 |
| NODE_2958 | Transketolase family protein | Pentose phosphate pathway | 45.9 | 242 | 239 |
| NODE_418 | Coronin | Phagocytosis | 75.9 | 226 | 252 |
| NODE_1833a | Lysozyme | Bacterial cell wall degradation | 70.6 | 134 | 434 |
| NODE_2008a | Lysozyme | 64.8 | 126 | 462 | |
| NODE_589 | Galactokinase family protein | Galactose metabolism | 65.2 | 71 | 800 |
| NODE_864 | Succinyl-CoA ligase beta-chain | Hydrogenosome metabolism | 78.1 | 60 | 946 |
a
Annotation for the top T. vaginalis hit was uninformative, and so a lower hit within the top 10 hits was selected.