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. 2023 Apr 12;14:2052. doi: 10.1038/s41467-023-37573-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b The Fractional Inhibitory Concentration (FIC) index for BRI + CAS in the ΔmpkA and ΔcalA mutant strains. The results are the average of three independent experiments. c Chitin synthase mRNA accumulation in A. fumigatus wild-type, ΔmpkA and ΔcalA strains. A. fumigatus strains were grown for 24 h at 37 °C and exposed or not to CAS [0.2 µg/mL CAS] combined or not with different concentration of BRI for 1 h. The results are the average of three independent experiments analyzed in technical duplicates each. d Exposure of chitin and as measured by Calcofluor White (CFW). The results graphic shows the value of nine technical replicates from three independent experiments ±SD deviation (n = 3; 2way ANOVA, Dunnet´s post-test; *p < 0.05 and ****p < 0.0001).