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. 2023 Apr 12;14:2052. doi: 10.1038/s41467-023-37573-y

Fig. 5. BRI can synergize CAS in other human fungal pathogens.

Fig. 5

a Metabolic activity expressed by XTT of C. neoformans grown for 48 h in the absence or presence of CAS [0 to 32 µg/mL] or BRI [0.625 µM] + CAS [0–32 µg/mL]. Percentage of survival expressed as colony forming units/ml of C. neoformans cells grown for 48 h in the absence or presence of CAS [0 to 16 µg/mL] or BRI [0.625 µM] + CAS [0–16 µg/mL]. Experiments were carried out in three repeats for each sample and three independent experiments (n = 3). b Metabolic activity expressed by XTT of C. albicans grown for 48 h in the absence or presence of CAS [0–1 µg/mL] or BRI [20 µM] + CAS [0 to 1 µg/mL]. Percentage of survival expressed as colony forming units/mL of C. albicans cells grown for 48 h in the absence or presence of CAS [0–1 µg/mL] or BRI [20 µM] + CAS [0–1 µg/mL]. Experiments were carried out in three repeats for each sample and three independent experiments (n = 3). c Metabolic activity expressed by XTT of C. albicans CAS-resistant strains grown for 48 h in the absence or presence of CAS [0.5 µg/mL], BRI [5–20 µM] or BRI [5 to 20 µM] + CAS [0.5 µg/mL]. Experiments were carried out in three independent replicates (n = 3), with three technical replicates and by analysed by Two-way ANOVA followed by Dunnett´s multiple comparison test (*p < 0.05 and ****p < 0.0001). The graphic shows the average of the nine repetitions ±SD. d Metabolic activity expressed by XTT of C. auris grown for 48 h in the absence or presence of CAS [0.125 µg/mL], BRI [10 µM] or BRI [10 µM] + [CAS 0.125 µg/mL]. Metabolic activity expressed by XTT of C. auris 467/2015 strain grown for 48 h in the absence or presence of CAS [0 to 1 µg/mL] or BRI [10 µM] + CAS [0 to 1 µg/mL]. Percentage of survival expressed as colony forming units/ml of C. auris 467/2015 strain grown for 48 h in the absence or presence of CAS [0 or 1 µg/mL] or BRI [10 µM] + CAS [0 to 1 µg/ml]. Experiments were performed in three independent biological replicates (n = 3), with three technical replicates and analysed by Two-way ANOVA followed by Dunnett´s multiple comparison test (*p < 0.05 and ****p < 0.0001). The graphic shows the average of nine repetitions ±SD.