Table 2.
Brilacidin MICs for human pathogenic fungi
| Species | MIC CAS (µg/ml) | FKS1 mutation | MIC BRI (µM) | Mechanism |
|---|---|---|---|---|
| Cryptococcus neorformans H99 | 32.0 | ND | 2.5 | Fungicidal |
| Candida albicans SC5314 | 0.25 | ND | 80 | Fungicidal |
| Candida albicans DPL1006 CASR | 2.0 | F641L | 20 | Fungicidal |
| Candida albicans DPL1007 CASR | 4.0 | F641S | 80 | Fungicidal |
| Candida albicans DPL1009 CASR | 4.0 | S645Y | 20 | Fungicidal |
| Candida albicans DPL1010 CASR | 2.0 | S645F | 80 | Fungicidal |
| Candida albicans DPL1011 CASR | 4.0 | S645F + R1361R/H | 40 | Fungicidal |
| Candida auris 467/2015 | 1.0 | ND | 80 | Fungicidal |
| Candida auris 468/2015 | 1.0 | ND | 80 | Fungicidal |
| Candida auris 469/2015 | 1.0 | ND | 80 | Fungicidal |
| Candida auris 470/2015 | 1.0 | ND | 80 | Fungicidal |
| Candida auris 471/2015 | 1.0 | ND | 80 | Fungicidal |
| MIC POSA (µg/ml) | CYP51 mutation | MIC BRI (µM) | Mechanism | |
| Rhizopus oryzae 99–892 | 2.0 | ND | 32 | Fungicidal |
| Rhizopus delemar 99–880 | 2.0 | ND | 32 | Fungicidal |
| Lichtheimia corymbifera | 1.0 | ND | 8.0 | Fungicidal |
| Mucor circineloides 131 | >16 | ND | 16 | Fungicidal |
Obs: Fungicidal activity was measured by plating the cells or conidia after 48 h incubation with the different drug concentrations on solid minimal medium and calculating the colony forming units divided by the initial inoculum; ND Not determined.