Reagent or Resource	Source	Identifier
Antibodies
Mouse monoclonal anti‐Gαi‐GTP	Graeme Milligan (Lane et al, 2008)	26901
Rabbit anti‐Arf1	Paul Randazzo (Marshansky et al, 1997)	n/a
Rabbit anti‐Mannosidase (Man)‐II	Gift from K. Moreman (Velasco et al, 1993)	n/a
Anti‐GFP	Living Colors, Invitrogen (Thermo Scientific)	Catalog # MA5‐15256
Anti‐RFP	Invitrogen (Thermo Scientific)	Catalog # MA5‐15257
Anti‐GIV coiled coil antibody	Millipore (Sigma)	ABT80
Goat anti‐Rabbit IgG, Alexa Fluor 594 conjugated	Thermo Fisher Scientific	A11072
Goat anti‐Mouse IgG, Alexa Fluor 488 conjugated	Thermo Fisher Scientific	A11017
IRDye 800CW Goat anti‐Mouse IgG Secondary (1:10,000)	LI‐COR Biosciences	926‐32210
IRDye 680RD Goat anti‐Rabbit IgG Secondary (1:10,000)	LI‐COR Biosciences	926‐68071
Biological samples
N/a
Chemicals, peptides, and recombinant proteins
DAPI (4′,6‐Diamidino‐2‐Phenylindole, Dilactate)	Thermo Fisher Scientific	D3571
MTT	Millipore Sigma	475989‐1GM
Puromycin	Sigma	P9620‐10ML
Brefeldin A	Sigma	B6542‐5MG
Fetal Bovine Serum	PEAK SERUM	PS‐FB1
Paraformaldehyde 16%	Electron Microscopy Biosciences	15710
Glutathione Sepharoseâ 4B	Sigma–Aldrich	GE17‐0756‐04
Protease inhibitor cocktail	Roche	11 873 580 001
Tyr phosphatase inhibitor cocktail	Sigma–Aldrich	P5726
Ser/Thr phosphatase inhibitor cocktail	Sigma–Aldrich	P0044
PVDF Transfer Membrane, 0.45 mM	Thermo Scientific	88518
Prolong Glass	Thermo Fisher Scientific	P36980
Paraformaldehyde 16%	Electron Microscopy Biosciences	15710
Guava Cell Cycle Reagent	Millipore Sigma	4700‐0160
Commercial kits
Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI)	Thermo Fisher Scientific	V13241
Experimental models: Cell lines
HeLa parental	ATCC	ATCC® CCL‐2
HeLa GIV KO (CRISPR Cas9)	Prior work (Abd El‐Hafeez et al, 2023)	n/a
MDA‐MB‐231	ATCC	ATCC® HTB‐26
MDA‐MB‐231 parental and GIV KO (CRISPR Cas9) lines	Prior work (Abd El‐Hafeez et al, 2023)	n/a
HeLa shControl	Prior work (Lo et al, 2015; Lopez‐Sanchez et al, 2015; Rohena et al, 2020)	n/a
HeLa shGIV	Prior work (Rohena et al, 2020)	n/a
Cos7 shControl	Prior work (Ma et al, 2015; Rohena et al, 2020)	n/a
Cos7 shGIV	Prior work (Ma et al, 2015; Rohena et al, 2020)	n/a
COS7	ATCC	ATCC® CRL‐1651™
HEK293T	ATCC	ATCC® CRL‐1573™
Recombinant DNA
Internally tagged Gαi1‐YFP	Moritz Bünemann (Bunemann et al, 2003; Gibson & Gilman, 2006; Lo et al, 2015; Midde et al, 2015)	N/A
Girdin CRISPR/Cas9 KO Plasmid (h2)	Santa Cruz Biotechnology (SCBT) Inc.	Sc‐402236‐KO‐2
CFP‐Gβ1	Lo et al (2015)	N/A
Temperature sensitive (ts)VSVG‐eGFP	Lo et al (2015)	N/A
MMP2‐GFP	Marc Coppolino (Kean et al, 2009)	N/A
MMP9‐GFP	Marc Coppolino (Kean et al, 2009)	N/A
Col VII‐RFP	Anderzej Fertala (Chung et al, 2009)	N/A
GST GAT (GGA)	Stuart Kornfeld (Dell'Angelica et al, 2000)	N/A
Other: Software
ImageJ	National Institute of Health	https://imagej.net/Welcome
IX81 FV1000 inverted confocal laser scanning microscope	Olympus	n/a
ClueGO	Cytoscape	Bindea et al (2009)
NetworkX	Python	https://networkx.org
Gephi	Gephi	https://gephi.org
Prism	GraphPad	https://www.graphpad.com/scientific‐software/prism/
LAS‐X	Leica	www.leica‐microsystems.com/products/microscope‐software/p/leica‐las‐x‐ls
Illustrator	Adobe	https://www.adobe.com/products/illustrator.html
MATLAB	MathWorks	https://www.mathworks.com/
ImageStudio Lite	LI‐COR	https://www.licor.com/bio/image‐studio‐lite/