Fig. 3.

The activation of the NF-κB signaling pathway is disrupted by the inhibition of RNF168 expression in podocytes of LN mice. a Protein expression of p-p65 (phospho S536) and t-p65 in the podocytes assessed by Western blot analysis. b Levels of TNF-α and IL-1β in the culture medium of podocytes assessed by ELISA. c Immunofluorescence staining of RNF168 and p-p65 (phospho S536) translocation into the nuclei of the podocytes. Cell experiment was repeated 3 times independently. *p < 0.05 versus the podocytes from MRL/MpJ mice or podocytes transfected with NC-shRNA plasmids.