Figure 2.

Results from analyzing the output files from Basic Protocol 3 (ntLink Option B) using QUAST. The scaffold NGA50 length (A) and misassembly (B) statistics for each round are shown. Running additional rounds of ntLink results in a more contiguous final assembly (Scaffold NGA50 length of 3.9 Mbp and 5.4 Mbp for round 1 and 2, respectively). The third round of scaffolding did not produce any additional joins, showing that further ntLink rounds would not benefit the assembly. More rounds may be required when the baseline assembly is more fragmented, or a larger genome is being scaffolded.