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Expected results from scaffolding the C. elegans Flye assembly using ntLink with the steps documented in Basic Protocol 1. Compared to the baseline, scaffolding using ntLink with overlap detection increases the assembly NGA50 length approximately 1.7-fold, while also reducing the number of misassemblies.
| Assembly | Number of sequences >= 3 kbp | NG50 length (Mbp) | NGA50 length (Mbp) | Number of misassemblies |
|---|---|---|---|---|
| Baseline assembly | 63 | 3.6 | 2.3 | 75 |
| Baseline assembly + ntLink | 33 | 6.8 | 3.7 | 66 |
