Fig. 5.

Sequential therapy of FAP-targeted and CLDN18.2-targeted CAR-T cells dramatically increase the accumulation of CD8⁺ T and CAR-T cells. a–c Tumor tissues were harvested from the mice at the end of therapy in the PANC02 model. a The representative images of tumor-infiltrating T cells immunostained with anti-CD4 and anti-CD8 from each treatment group. b Histograms show the quantification of T cells in tumor tissues. c CAR copy numbers in genomic DNA of residual tumors from each treatment group were measured by qRT-PCR (TaqMan probe). d–f Tumor tissues were harvested from the mice at the end of therapy in the KPC1199 model. d The representative images of tumor-infiltrating T cells immunostained with anti-CD4 and anti-CD8 from each treatment group. e Histograms show the quantification of T cells in tumor tissues. f CAR copy numbers in genomic DNA of residual tumors from each treatment group were measured by qRT-PCR (TaqMan probe). The images were obtained under original magnification 200×. Scale bars, 100 μm. All data are presented as the mean ± SEM of triplicate experiments. *p < 0.05, **p < 0.01