Fig. 3.

Effect of α-LA supplementation on PANK2 gene expression. Control and PKAN fibroblasts (P1, P2, P3) were treated with lipoic acid at 10 μM for twenty days. a PANK2 transcripts were quantified by RT-qPCR. b NF-Y, FOXN4 and hnRNPA/B transcription factors expression levels were analyzed by Western blotting. Actin was used as a loading control. c Densitometry of Western blotting. *p < 0.05, **p < 0.01, ***p < 0.005 ****p < 0.001 between PKAN patients and controls. ^#p < 0.05, ^##p < 0.01, ^###p < 0.005 between untreated and treated fibroblasts. A.U., arbitrary units