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. 2023 Apr 12;21:128. doi: 10.1186/s12951-023-01869-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — MiR-222-3p directly targets Bim. A Venn diagram showing the number of genes. significantly upregulated in ATMs from obese donors compared with ATMs from lean donors according to microarray analysis and the number of potential targets of miR-222-3p predicted by target prediction algorithms. B Enrichment analysis of pathways performed by Metascape. C Predicted binding sites between miR-222-3p and the Bim 3'-UTR. D Dual-luciferase assays were performed in HEK293T cells after co-transfection with Bim 3'-UTR wild-type (WT) or mutant (MUT) plasmids and miR-222-3p mimic or miR-NC mimic. ^*P < 0.05 compared with the WT group by repeated-measures analysis of variance (n = 3). E, F Western blotting was used to analyze Bim and β-actin protein levels in RAW264.7 cells treated with LPS, LPS + Exos^Lean, LPS + Exos^Lean + miR-222-3p inhibitor, or LPS + Exos^Lean + miR-NC inhibitor. ^*P < 0.05, ^#P < 0.05 by repeated-measures analysis of variance (n = 3)