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. 2023 Apr 12;21:128. doi: 10.1186/s12951-023-01869-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Overexpression of Bim inhibited macrophage polarization. A Bim mRNA expression was measured by qRT‒PCR. B, C Western blotting was used to analyze Bim and β-actin protein levels. ^*P < 0.05 by repeated-measures analysis of variance (n = 3). D Flow cytometry analysis and quantification of the relative abundance of CD86+ cells. E Flow cytometry analysis and quantification of the relative abundance of CD206+ cells. F, G Immunofluorescence staining image showing the localization of iNOS + cells and statistical analysis of the proportion of these cells. (H, I) Immunofluorescence staining image showing the localization of Arg1 + cells and statistical analysis of the proportion of these cells. Scale bars: 50 μm; ^*P < 0.05, ^#P < 0.05 by repeated-measures analysis of variance (n = 3)