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. 2022 Oct 10;79(1):284–294. doi: 10.1002/ps.7198

Figure 5.

Figure 5

(a) Pea aphid survival 24 h postcontact exposure to water, water+ Breakthru (BT), HxTx‐Hv1h (HxTx), GNA, HxTx‐Hv1h/GNA (FP), or a mixture of GNA and toxin. All protein treatments contained BT. Bars depict standard error of the mean (three replicates of n = 15 per dose). Significant differences to control (+BT) (by Student's t‐tests): *, P < 0.05; **, P < 0.005; ***, P < 0.0005. (b) Representative images of aphids taken 18 h postcontact with droplets containing 70 pmol FITC‐labelled HxTx‐Hv1h (HxTx), GNA or HxTx‐Hv1h/GNA (FP). Aphids were fed on control diet and washed before imaging under FITC filter captured in openlab. Scale bar = 0.5 mm. (c) Western analysis (anti‐His antibodies) of whole aphid protein samples extracted 2 h and 18 h postcontact with droplets containing HxTx‐Hv1h, GNA or fusion protein. S1, HxTx‐Hv1h standard (250 ng); S2, fusion protein (200 ng); and S3, recombinant GNA (50 and 100 ng).