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. 2023 Mar 13;13(6):1809–1822. doi: 10.7150/thno.83082

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Calhm2 interacts with EFhd2. (A) Flow chart of cells collected, immunoprecipitation of Flag, silver stained, and mass spectrometry analysis in BV2 control cells and overexpressed Calhm2 cells; (B) Silver staining results of immunoprecipitation of Flag samples for proteins. (C) Mass spectrometry results analyzing protein peptide segment differences within control and overexpressed Calhm2 cells. (D) Pattern diagram of Calhm2-HA mouse constructs. (E) Immunoprecipitation using Calhm2 WT with Calhm2 HA mouse brain tissues, and mass spectrometry results analyzing Calhm2 HA interacting proteins. (F) Co-transfection of Myc-EFhd2 and HA-Calhm2 plasmids in HEK293T cells, LPS stimulation for 6 h, and immunoblotting for Myc and HA after immunoprecipitation of HA.