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. 2023 Mar 13;13(6):1809–1822. doi: 10.7150/thno.83082

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Calhm2 regulates the phosphorylation of EFhd2 and downstream STAT3 signaling in microglia. (A) Diagram of the experimental model. (B) Expression of EFhd2 in control and Calhm2 knockdown cells by immunoblotting after LPS stimulation of BV2 normal control cells and knockdown Calhm2 cells at different time points. (C) Immunoprecipitation of phosphotyrosine and immunoblot detection of EFhd2 in BV2 normal control cells and knockdown Calhm2 cells after LPS stimulation for 6 h, (D) Diagram of the experimental model. (E) Expression of EFhd2, p-STAT3 and STAT3 in control and Calhm2 knockdown cells by immunoblotting after LPS stimulation at different time points. (F) Expression of EFhd2, p-STAT3 and STAT3 in control and EFhd2 knockdown cells by immunoblotting after LPS stimulation at different time points.