Figure 6.

(A) a) Synthesis of etched HIONCs and the encapsulation of GOD into HIONCs. b) GOD-induced starvation chemodynamic therapy for enhanced magnetic hyperthermia therapy in the hypoxic tumor microenvironment. HIONC-mediated oxygen replenishment simultaneously enhanced starvation therapy. (B) Quantitative temperature curve of ultrapure water and HIONCs in vitro: a) microcentrifuge tube, b) gel, and c) cattle liver. d) Heating curves of the HIONCs-GOD dispersion in water (10 mg/mL) exposed to the AMF of 513 kHz at 1001.1 A/m for seven on/off cycles. e) Thermal images of PC3 tumor-bearing mice after exposure to the AC magnetic field, including HIONCs-GOD + AMF, HIONCs + AMF, and PBS + AMF. (f) Temperature curve against time for the HIONCs-GOD, HIONC, and phosphate-buffered saline (PBS) groups in vivo. The temperature changes were recorded with a thermal infrared camera. (C) (a) Relative tumor volume after treatment. The animals were monitored every other day (n = 5, *P < 0.05, **P < 0.01). (b) Relative body weight of mice in the different groups after treatment. (c) Survival curves of different groups during the 21 d observation period. Representative images of PC3 tumor tissue sections at 24 h after treatment with PBS, PBS + AMF, free GOD, HIONCs-GOD, HIONCs, HIONCs + AMF, or HIONCs-GOD + AMF: immunofluorescence staining with (d) anti-HSP70 and (e) anti-HSP90 antibody, (f) H&E, and (g-i) immunohistochemical staining. Green: HSP70 and HSP90 expressing region. The white scale bar in (d-f) is 200 μm. (g) TUNEL, (h) caspase 9, (i) caspase 3. The black scale bar in (g-i) is 100 μm. Reproduced with permission ¹⁴⁸. Copyright 2020, American Chemical Society.