Skip to main content
. 2023 Mar 27;13(6):2040–2056. doi: 10.7150/thno.80555

Figure 1.

Figure 1

An increase in platelets can promote the development of tumors. (A) In vivo model of high platelet counts induced by four subcutaneous (s.c.) TPO injections. (B) Tumor growth was monitored (n = 10). (C) Tumor weights were examined (n = 10). (D) The increased platelet model established by six platelet transfusions. (E) Tumor growth in mice with or without platelet transfusion was monitored (n = 9). (F) The tumor weights were examined (n = 9). (G) Tumor growth in syngeneic CRC models established in Aurkafl/fl and Aurkafl/fl;Cd19cre/+ mice was monitored (n = 8). (H) Tumor weights were examined (n = 8). (I) Aurkafl/fl and Aurkafl/fl;Cd19cre/+ mice were transfused with the control or B cells. Tumor volumes and tumor weights were examined (n = 9). (J) Tumor growth in Rag2-/- mice transfused with or without platelets was monitored. Tumor weights were examined (n = 9). (K-L) Tumor-bearing mice were treated with the control or clopidogrel. The tumor growth and tumor weights were examined (K: n = 6, L: n = 7). (M) Survival was monitored 24 days after MC-38 injection. Statistical analysis of survival was performed with a log-rank test (n = 8). (N) Representative images of India ink-stained lungs with arrowheads indicating metastatic nodules (n = 3/ time point). (O) Representative H&E images of lung sections from Aurkafl/fl and Aurkafl/fl;Cd19cre/+ mice injected with MC-38 cells. Scale bar, 2 mm, (n = 3). **P < 0.01; *P < 0.05; n.s., not significant.