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. 2023 Mar 27;13(6):2040–2056. doi: 10.7150/thno.80555

Figure 3.

Figure 3

Elevated platelet-regulated TAM education by the C5a/C5aR1 axis. (A) The serum concentration of C5a was measured by ELISA (n = 5). The data shown are representative of one of two independent experiments. (B) The level of intratumoral C5aR1+ TAMs was examined by FACS (n = 5). (C) Real-time RT‒PCR was used to quantify the levels of C5 and C5ar1 mRNA (n = 3). (B-C) The data shown are representative of a single experiment. (D) The serum concentration of C5a was measured by ELISA (n = 5). The data shown are representative of one of two independent experiments. (E) The level of intratumoral C5aR1+ TAMs was examined by FACS (n = 9). (F) Real-time RT‒PCR was used to quantify the levels of C5 and C5ar1 mRNA (n = 3). (E-F) The data shown are representative of a single experiment. (G) The serum concentration of C5a was measured by ELISA (n = 7). The data shown are representative of a combination of two independent experiments. (H) The level of intratumoral C5aR1+ TAMs was examined by FACS (n = 7). (I) Real-time RT‒PCR was used to quantify the levels of C5 and C5ar1 mRNA (n = 3). (H-I) The data shown are representative of a single experiment. (J) Real-time RT-PCR was used to quantify the levels of C5 and Il10 mRNA after C5aR1 inhibition in vitro. The data shown are representative of one of two independent experiments. (K-L) Tumor volumes and tumor weights were examined (K: n = 7; L: n = 4). The data shown are representative of a single experiment. **P < 0.01; *P < 0.05; n.s., not significant.