TABLE 1.
Primers used in RT‐qPCR analyses
| Gene | (+) Forward primer sequence | Gene accession number | Amplicon (bp) |
|---|---|---|---|
| (−) Reverse primer sequence | |||
| Kcnq1 | TGGGTCTCATCTTCTCCTCC | NM_032073 | 124 |
| GTAGCCAATGGTGGTGACTG | |||
| Kcnq2 | AAGAGCAGCATCGGCAAAAA | NM_133322 | 101 |
| GGTGCGTGAGAGGTTAGTAGCA | |||
| Kcnq3 | CAGCAAAGAACTCATCACCG | AF091247 | 161 |
| ATGGTGGCCAGTGTGATCAG | |||
| Kcnq4 | GAATGAGCAGCTCCCAGAAG | XM_233477.8 | 133 |
| AAGCTCCAGCTTTTCTGCAC | |||
| Kcnq5 | AACTGATGAGGAGGTCGGTG | XM_001071249.3 | 120 |
| GATGACCGTGACCTTCCAGT | |||
| Kcne1 | GTTTCCCCAAATCTCTCCATT | NM_008424.3 | 111 |
| AGCACACACTTCCCATTTCAA | |||
| Kcne2 | CCTGGTATTTAACTGAGTTGGACAT | NM_133603.2 | 97 |
| GCACTGGGGGCTCTTGAAT | |||
| Kcne3 | CTCAACCATATCAAGCCACAGT | NM_022235.2 | 99 |
| GCCTATCAGTCCCTCTTCTCT | |||
| Kcne4 | GGAGGAGGGGGCTGATGA | NM_212526.1 | 88 |
| CTGGTGGATGTTCTCGGAAGA | |||
| Kcne5 | GCACGAAGAGACCTCAGACAT | NM_001101003.1 | 146 |
| GGACAGGAAACAAGAACACCAT | |||
| Esr1 | TTCACCTTCTGGAGTGTGCC | NM_012689.1 | 173 |
| ACTTGACGTAGCCAGCAACA | |||
| Esr2 | TGCCGACTTCGCAAGTGTTA | NM_012754.2 | 138 |
| ACCGTTTCTCTTGGCTTTGC | |||
| Gper1 | TCATCGGCCTGTGCTATTCC | NM_133573 | 119 |
| GAAGACAAGGACCACTGCGA | |||
| Cd31 | CTCCTAAGAGCAAAGAGCAACTTC | NM_031591.1 | 100 |
| TACACTGGTATTCCATGTCTCTGG | |||
| Acta2 | ATCCGATAGAACACGGCATC | NM_031004.2 | 228 |
| AGGCATAGAGGGACAGCACA |